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Objective:To investigate the variation characteristics and influencing factors of HIV/AIDS subtypes in Wuxi city of Jiangsu Province from 2014 to 2016.Methods:HIV/AIDS population in Wuxi city in 2014 was selected as the research object, and the HIV molecular epidemiology and follow-up study were carried out. Collect epidemiological information, extract DNA from blood samples, amplify pol gene fragment by nest-PCR and sequence, use ChromasPro 1.6 software and MEGA 7.0 software to construct the HIV-1 sequence database, and use FastTree2.1.10 software to construct the phylogenetic tree to confirm the subtype; in 2016, the same population was followed up, and the HIV subtype variation was analyzed, and the influencing factors of subtype variation were explored by multivariate logistic regression. Results:A total of 612 HIV/AIDS cases in 2014 and 2016 were collected. The age of the subjects was mainly 30 years old or above (85.46%, 523/612), and the proportion of people over 50 years old was higher (228/612, 37.25%). The main route of transmission was homosexuality, accounting for 49.67%. A total of 1224 samples were detected and CRF01 _ AE、CRF07_ BC、B、CRF08_ BC、CRF67_ 01B、CRF55_ 01B、CRF68_ 01B, 7 subtypes of HIV-1 and 5 unique recombinant types (URFs) was detected. CRF01_ AE and CRF07_ BC was still the main genotype in Wuxi, Jiangsu Province, accounting for 66.75%. There were 29 cases (3.56%) of URFs recombinant strains. During 2014-2016, the variation rate of subtypes was 14.63%, and the most common variation was CRF01_ AE changes to CRF07_ BC(13.95%). Marital status (OR=0.363, 95% CI: 0.137-0.964) and baseline CD4 level (OR=0.414, 95% CI: 0.192-0.891) were associated with subtype variation.Conclusions:The HIV-1 subtypes of HIV/AIDS patients in Wuxi city are diverse and complex, the proportion of recombinant subtypes is rising, the URFs that are difficult to determine the genotype increase significantly, and the variation rate of HIV-1 subtypes among HIV/AIDS infected people is high. It is necessary to strengthen the monitoring of HIV-1 subtypes.
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Objective@#To assess the prevalence and related factors of HIV infection among male clients of the female sex workers in Hekou Yao autonomous county of Honghe Hani Yi autonomous prefecture (Hekou county) in Yunnan province in China, 2014-2015.@*Methods@#Serial cross-sectional survey was conducted during June 2014 to November 2015. Convenience sampling methods were used to recruit the male clients for this study. Self-reported information on social-demographic characteristics, with sexual and drug behavior patterns, was gathered. Both blood and urine samples were collected for HIV, with for opiate testing. Multivariate logistic regression and Exhaustive CHAID method were used to determine the correlated factors associated with HIV infection. Statistical analysis was used by SPSS 22.0 software and Clementine 12.0 software.@*Results@#The overall HIV prevalence of male clients was 2.06% (16/776). Male clients who keep using condom with female sex worker was estimated as 68.81% (534/776). The last commercial sexual partner of Vietnamese male clients was all Vietnamese female sex workers. Compared with Chinese male clients, Vietnamese male clients have a higher rate of morphine positive. Factors as: age ≥50 years vs. age <30 years (OR=8.11, 95%CI: 1.26-52.16) and testing for morphine positive vs. morphine negative (OR=7.35, 95%CI: 1.42-38.06) were significantly associated with HIV infection through multiple logistic regression analysis. Through Exhaustive CHAID, it confirmed that age was the primary factor that associated with HIV infection of male clients.@*Conclusions@#Relationship between morphine and HIV infection indicated that HIV prevalence of male clients in Hekou county was influenced by the combined effect of both illegal drug use and commercial sexual behavior. Special attention should be paid to male clients over 50 years of age, on HIV intervention.
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Objective To assess the relationship between HIV genetic subtypes and HIV resistance in China.Methods The literature retrieval was conducted by using Chinese ScienceTechnology Journal Database (VIP),Wanfang Data,Chinese Journal Full-text Database (CNKI),PubMed and Web of Science to select the papers on the relationship between HIV subtypes and HIV drug resistance in China during 2005-2015.Eligible papers were included according to the inclusion.Meta-analysis was performed by using software Stata 12.0.Results A total of 43 papers were selected and the pooled rate of drug resistance was 15.1％ and the rate of primary drug resistance was 9.5％,the subtypes associated drug resistance were CRF01_AE,CRF07 BC,CRF08_ BC,B/B'and C.The pooled rates of drug resistance of each subtype were 12.8％,7.4％,14.3％,25.7％ and 34.9％ and the rates of primary drug resistance of each subtype were 7.3％,5.7％,11.5％,15.5％ and 23.9％,respectively.Subgroup analysis showed that both treated and area subgroup showed a significant difference among groups (P＜0.05).The rates of primary resistance of each subtype in northern China and southwestern China were higher than that in southern China.Conclusion The distribution of HIV genotypes in China was complex and the prevalence of primary drug resistance of each subtype was high,together with a significant difference among subtypes.It is necessary to strengthen the monitoring of different subtypes of drug resistant strains in China to prevent the recombination and spreading of resistant strains.
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Objective To assess the relationship between HIV genetic subtypes and HIV resistance in China.Methods The literature retrieval was conducted by using Chinese ScienceTechnology Journal Database (VIP),Wanfang Data,Chinese Journal Full-text Database (CNKI),PubMed and Web of Science to select the papers on the relationship between HIV subtypes and HIV drug resistance in China during 2005-2015.Eligible papers were included according to the inclusion.Meta-analysis was performed by using software Stata 12.0.Results A total of 43 papers were selected and the pooled rate of drug resistance was 15.1％ and the rate of primary drug resistance was 9.5％,the subtypes associated drug resistance were CRF01_AE,CRF07 BC,CRF08_ BC,B/B'and C.The pooled rates of drug resistance of each subtype were 12.8％,7.4％,14.3％,25.7％ and 34.9％ and the rates of primary drug resistance of each subtype were 7.3％,5.7％,11.5％,15.5％ and 23.9％,respectively.Subgroup analysis showed that both treated and area subgroup showed a significant difference among groups (P＜0.05).The rates of primary resistance of each subtype in northern China and southwestern China were higher than that in southern China.Conclusion The distribution of HIV genotypes in China was complex and the prevalence of primary drug resistance of each subtype was high,together with a significant difference among subtypes.It is necessary to strengthen the monitoring of different subtypes of drug resistant strains in China to prevent the recombination and spreading of resistant strains.